منابع مشابه
Kinetic studies of rabbit muscle lactate dehydrogenase.
A number of reports have appeared in recent years on the mechanism, i.e. the mode of enzyme and substrate interaction, of anaerobic dehydrogenases. For example, the mechanisms of heart muscle lactate dehydrogenase (1)) yeast alcohol dehydrogenase (2), and ribitol dehydrogenase (3) appear to involve a compulsory sequence of enzyme and substrate interaction leading to one or more kinetically impo...
متن کاملKinetic studies of dogfish liver glutamate dehydrogenase.
Initial-rate studies were made of the oxidation of L-glutamate by NAD+ and NADP+ catalysed by highly purified preparations of dogfish liver glutamate dehydrogenase. With NAD+ as coenzyme the kinetics show the same features of coenzyme activation as seen with the bovine liver enzyme [Engel & Dalziel (1969) Biochem. J. 115, 621--631]. With NADP+ as coenzyme, initial rates are much slower than wit...
متن کاملKinetic studies of liver alcohol dehydrogenase.
1. NADH2 prepared by enzymic reduction of pure NAD by the method of Rafter & Colowick (1957), and isolated as the sodium salt, gives higher maxrimum rates of reduction of acetaldehyde with liver alcohol dehydrogenase at pH 6 than a number of commercial preparations of high purity. Maximum values of 2-4 for the extinction ratio E260/E340 and 2% for the proportion of inactive material absorbing a...
متن کاملProtein engineering of formate dehydrogenase.
NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is one of the best enzymes for the purpose of NADH regeneration in dehydrogenase-based synthesis of optically active compounds. Low operational stability and high production cost of native FDHs limit their application in commercial production of chiral compounds. The review summarizes the results on engineering of bacterial and yeast FDHs a...
متن کاملSome properties of formate dehydrogenase.
In addition the enzyme catalyzes the oxidation of NADH by 0 2 . The stoichiometry of the first reaction is proved by the formation of the amount of NADH which under anaerobic conditions exactly corresponds to the amount of formate used. In this reaction NAD is replaceable by a large number of redox dyes. For the second reaction the stoichiometry is proved a) kinetically by the relation of the v...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1200763